This article from Thermo Fisher Scientific explores how Amira software can be used as a novel approachfor visualizing the developing skeleton in 3D.
Sponsored Content by Thermo Fisher Scientific - SoftwareNov 8 2023Reviewed by Emily Magee For years, the standard method for imaging the development of vertebrate skeletons has been the use of Alizarin Red and Alcian Blue for clearing and staining.1 These techniques have also been applied in the study of whole-embryo skeletal development.
Refining the process The process has been refined through the utilization of optical projection tomography, which effectively 3D-images Alcian Blue stained cartilage in cleared specimens.3 Their innovative approach involves selectively labeling the cartilage matrix, providing adequate contrast compared to other tissues for automated visualization and segmentation while preserving the bone mineral. This enables simultaneous imaging of both tissues in the developing skeleton.
They successfully obtained morphometric measurements for these skeletal components. However, a challenge they faced was the overlap in X-Ray attenuation between fetal bone and cartilage. This achievement marked the first instance of a high-contrast staining protocol that facilitated both intensity-based segmentation and visual phenotyping of cartilage elements, which can be used for quantitative and qualitative analyses of the cartilage developmental process.1
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